![]() A total of 150 strains per culture medium were then identified using classical methods.Īmong the isolates from MRS agar, Lactobacillus sakei predominated (23.3% of the isolates), followed by Lactobacillus alimentarius (17.3%), Lactobacillus curvatus (15.3%), Lactobacillus plantarum (12%) and Lactobacillus farciminis (10%). In general, high counts of all the investigated microbial groups were observed, with average values of 8.65 log Colony Forming Units (CFU)/g for the total aerobic mesophilic flora, 8.87 log CFU/g for the lactic acid bacteria, 6.56 log CFU/g for the salt tolerant flora, and 2.98 log CFU/g for the Enterobacteriaceae.Īfter the end of the incubation period, from MRS agar, SPC agar +7.5% NaCl, and VRBG agar, 10 colonies were randomly taken from each Botillo unit and from each culture medium. ![]() ![]() Counts of total aerobic mesophilic flora on Standard Plate Count (SPC) agar, lactic acid bacteria on de Man Rogosa Sharpe (MRS) agar, salt tolerant flora on SPC agar +7.5% NaCl, and Enterobacteriaceae on Violet Red Bile Glucose (VRBG) agar, and some physico-chemical parameters (Total solids and NaCl contents and pH and a w values) were determined in 15 units of Botillo, a traditional dry-fermented sausage made in the NW of Spain.
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